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. 2017 Sep 8;18:170. doi: 10.1186/s12931-017-0653-3

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Analysis of CD4⁺/CD25⁺/Foxp3⁺ subpopulations from bronchoalveolar lavage (BAL) samples by cell-surface molecules and intracellular proteins. a Two subsets of Treg cells in BAL defined by the expression of CD45RA and Foxp3. Fraction II: CD45RA⁻Foxp3^high activated T-regulatory cells (aTregs); Fraction III: CD45RA⁻Foxp3^low cytokine-secreting cells. b The expression of CD25 in each fraction shown in (a). c Representative flow cytometric analysis of Treg subpopulations by the expression of CD45RA and Foxp3. d, e Percentages of Treg subpopulations among CD4⁺ T cells in the BAL of HCs and patients with pSS-IP or IPF