Table 1.
Genotype and allele frequencies of hsa-miR-499a seed region variant in asthma patients and controls
| Asthma | Controls | P value | Crude OR (95%CI) | Adjusted OR (95%CI) a | ||
|---|---|---|---|---|---|---|
| DISCOVERY STAGE | ||||||
| Total number | (n = 96) | (n = 96) | ||||
| Genotype frequency | ||||||
| Co-Dominant model | AA | 20 (20.8) | 56 (58.3) | <0.001 | 1.0 | 1.0 |
| AG | 38 (39.6) | 30 (31.3) | 3.54 (1.76–7.14) | 3.67 (1.74–7.77) | ||
| GG | 38 (39.6) | 10 (10.4) | 10.6 (4.48–25.2) | 10.9 (4.41–27.3) | ||
| Dominant model | AA | 20 (20.8) | 56 (58.3) | 1.0 | ||
| AG + GG | 76 (79.2) | 40 (41.7) | 5.32 (2.81–10.1) | |||
| P-HWE | 0.064 | 0.078 | ||||
| Allele frequency | ||||||
| Allelic model | A | 78 (40.6) | 142 (73.9) | <0.001 | 1.0 | |
| G | 114 (59.3) | 50 (26.04) | 4.1 (2.69–6.39) | |||
| Carriage rate | ||||||
| A | 58 (60.4) | 86 (89.6) | <0.001 | 0.17 (0.08–0.38) | 0.29 (0.12–0.70) | |
| G | 76 (79.2) | 40 (41.7) | <0.001 | 4.71 (2.52–8.80) | 3.22 (1.57–6.62) | |
| REPLICATION STAGE | ||||||
| Total number | (n = 115) | (n = 204) | ||||
| Genotype frequency | ||||||
| Co-Dominant model | AA | 14 (12.2) | 82 (40.2) | <0.001 | 1.0 | 1.0 |
| AG | 48 (41.7) | 95 (46.6) | 2.95 (1.52–5.75) | 2.79 (1.46–5.06) | ||
| GG | 53 (46.1) | 27 (13.2) | 11.4 (5.52–23.9) | 10.8 (4.65–20.3) | ||
| Dominant model | AA | 14 (12.2) | 82 (40.2) | <0.001 | 1.0 | |
| AG + GG | 101 (87.8) | 122 (59.8) | 4.84 (2.59–9.06) | |||
| P-HWE | 0.542 | 0.950 | ||||
| Allele frequency | ||||||
| Allelic model | A | 76 (33.1) | 259 (63.5) | <0.001 | 1.0 | |
| G | 154 (66.9) | 149 (36.5) | 3.52 (2.50–4.95) | |||
| Carriage rate | ||||||
| A | 62 (53.9) | 177 (86.8) | <0.001 | 0.17 (0.1–0.30) | 0.23 (0.2–0.34) | |
| G | 101 (87.8) | 122 (59.8) | <0.001 | 4.84 (2.59–9.06) | 4.79 (2.64–8.9) | |
| OVERALL ANALYSIS | ||||||
| Total number | (n = 211) | (n = 300) | ||||
| Genotype frequency | ||||||
| Co-Dominant model | AA | 34 (16.1) | 138 (46.0) | <0.001 | 1.0 | 1.0 |
| AG | 86 (40.8) | 155 (41.7) | 2.25 (1.42–3.56) | 2.13 (1.24–3.46) | ||
| GG | 91 (43.1) | 37 (12.3) | 9.98 (5.84–17.05) | 9.52 (5.61–16.5) | ||
| Dominant model | AA | 34 (16.1) | 138 (46.0) | |||
| AG + GG | 177 (83.9) | 162 (54.0) | <0.001 | 1.0 | ||
| P-HWE | 0.079 | 0.507 | 4.43 (2.88–6.82) | |||
| Allele frequency | ||||||
| Allelic model | A | 154 (36.5) | 431 (62.3) | <0.001 | 1.0 | |
| G | 268 (63.5) | 229 (34.7) | 3.27 (2.53–4.22) | |||
| Carriage rate | ||||||
| A | 120 (56.9) | 263 (87.7) | <0.001 | 0.18 (0.12–0.28) | 0.12 (0.08–0.21) | |
| G | 175 (82.9) | 162 (54.0) | <0.001 | 4.14 (2.71–6.33) | 3.96 (2.14–5.9e) | |
Values are presented as number (percentage). P-HWE, p values of Hardy-Weinberg equilibrium
aAdjusted for confounding variables (age, gender, residence, family history, BMI percentile, puberty) was done by binary logistic regression (Method = Enter). Chi-square test was used. Bold values indicate significance at p < 0.05