Table 1.
Summary of exon deletions generated by Cas9 RGN using electroporation and microinjection
| Target | Condition1 | Cas9 | Embryos | Live Born | NHEJ2 | Deletions |
|---|---|---|---|---|---|---|
| Pcnx2 (433 bp del) | EP-1X | mRNA | 80 | 34 | 2 (6%) | 0 (0%) |
| EP-6X | mRNA | 60 | 21 | 4 (19%) | 0 (0%) | |
| EP-1X | Protein | 80 | 31 | 1 (3%) | 0 (0%) | |
| EP-6X | Protein | 60 | 12 | 0 (0%) | 0 (0%) | |
| MIJ | mRNA | 75 | 18 | 8 (44%) | 7 (39%) | |
| MIJ | Protein | 70 | 21 | 8 (38%) | 7 (33%) | |
| Neil2 (481 bp del) | EP-1X | mRNA | 60 | 27 | 8 (30%) | 3 (11%) |
| EP-6X | mRNA | 57 | 14 | 8 (57%) | 2 (14%) | |
| EP-1X | Protein | 60 | 12 | 4 (33%) | 1 (8%) | |
| EP-6X | Protein | 54 | 6 | 1 (17%) | 4 (67%) | |
| MIJ | mRNA | 50 | 10 | 0 (0%) | 6 (60%) | |
| MIJ | Protein | 60 | 10 | 1 (10%) | 7 (70%) | |
| Rab6b (232 bp del) | EP-1X | mRNA | 44 | 8 | 1 (13%) | 3 (38%) |
| EP-6X | mRNA | 60 | 12 | 3 (25%) | 6 (50%) | |
| EP-1X | Protein | 51 | 8 | 3 (38%) | 3 (38%) | |
| EP-6X | Protein | 60 | 10 | 3 (30%) | 6 (60%) | |
| MIJ | mRNA | 56 | 18 | 1 (6%) | 15 (83%) | |
| MIJ | Protein | 54 | 12 | 1 (8%) | 10 (83%) |
1
Conditions tested were electroporation (EP) with program repeated one or six times (1X, 6X) versus microinjection (MIJ).
2
Percentages are based upon number of non-homologous (NHEJ) events or deletions obtained over total number of live born.