Table 2.
Rate constants of fluorescent tracer exchange between vegetative cells in Anabaena and indicated amiC mutant strains used in this work.
| Mean R (s−1) ± SD (n) | ||
|---|---|---|
| Anabaena strain | Calcein | 5-CF |
| PCC 7120 | 0.090 ± 0.018 (24) | 0.120 ± 0.029 (12) |
| SR477 | 0.056 ± 0.013 (9) | 0.038 ± 0.010 (33) |
| FQ1633 | 0.051 ± 0.015 (18) | 0.032 ± 0.005 (3) |
| FQ1633 SR478 | ||
| (normal filaments) | 0.057 ± 0.009 (5) | 0.039 ± 0.012 (7) |
| (aberrant filaments)* | 0.000 ± 0.000 (6) | 0.000 ± 0.000 (16) |
| SR477 DR1992 | 0.047 ± 0.008 (2) | 0.051 ± 0.002 (2) |
FRAP analysis was performed and evaluated as described in Materials and Methods.
Data are mean ± SD from the results obtained with the number n of filaments subjected to FRAP analysis in parenthesis.
39% of cells showed aberrant morphology (see also Table S3).