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. 2017 Sep 5;8:1697. doi: 10.3389/fmicb.2017.01697

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Copyright © 2017 Tian, Wang, Zhang, Luo, Jiang, Zhang, Mei, Wu, Wu, Peng, Long, Luo and Guo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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P of GD-SH-01 alone cannot induce detectable apoptosis. (A) Graphic illustration of constructed plasmids of mRFP-P, and control plasmid mRFP via seamless cloning technology on the background of mRFP-shP which was previously constructed. (B) NA cells were transfected with mRFP-shP, mRFP-P, and mRFP, respectively, and transfection without plasmid was used as a control. Apoptosis rates were determined 2 days post transfection by means of FCM. (C) The results in bar chart come from three scatter diagrams of each group. Data are represented as means ± SD of three independent experiments. One-way ANOVA followed by Bonferroni’s multiple comparison test. ^∗p < 0.05, ns, not significant. (D) The efficacy of the transfection and expression levels of P was examined under a fluorescence microscope at 2 days post-transfection (representative images were presented here).