Table 1.
Target | Primers | Primer sequence (5′–3′) | Length (bp) |
---|---|---|---|
Linearized vector | VP1 | CAGGCAACACCACTGATAAGATGAACTTCCTACGCAAGATAG | 1073 |
VP2 | AGTTTTTTTCATGTGAGATATACACAATTTGCAGGTTCTTTTCAATCTC | ||
Inserted fragment | FP1 | TATCTCACATGAAAAAAACTAACACTCCTCCTTTCGAACCATCCCAAGTATG | 15,717 |
FP2 | CTTATCAGTGGTGTTGCCTGTTTTTTTCATATCGACTCCATGACATCTCAG | ||
a20 bp homologous sequences are underlined.