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. 2017 Sep 5;8:1697. doi: 10.3389/fmicb.2017.01697

Table 1.

Sequence of primers used to assembly full-length genome cDNA of rGDSH-Pa.

Target Primers Primer sequence (5′–3′) Length (bp)
Linearized vector VP1 CAGGCAACACCACTGATAAGATGAACTTCCTACGCAAGATAG 1073
VP2 AGTTTTTTTCATGTGAGATATACACAATTTGCAGGTTCTTTTCAATCTC
Inserted fragment FP1 TATCTCACATGAAAAAAACTAACACTCCTCCTTTCGAACCATCCCAAGTATG 15,717
FP2 CTTATCAGTGGTGTTGCCTGTTTTTTTCATATCGACTCCATGACATCTCAG

a20 bp homologous sequences are underlined.