Figure 7.

Biofilm formation doesn't affect pyoverdine production in SK media. (A) Pyoverdine fluorescence normalized to bacterial growth measured kinetically over 24 h in biofilm mutants grown in SK media. (B) Difference in bacterial growth for bacteria grown in M9 or SK media measured by absorbance at 600 nm. (C) Difference in biofilm formation for bacteria grown in M9 or SK media measured by crystal violet biofilm matrix stain solubilized in acetic acid. (D) Pyoverdine fluorescence normalized to bacterial growth measured kinetically over 24 h in c-di-GMP biosynthesis mutants grown in SK media. All error bars represent SEM between four technical replicates. Asterisks indicate significant difference between conditions (p-value < 0.01, based on Student's t-test). Pyoverdine production curves without bacterial growth normalization are available in Figure S6.