Fig. 3.—

Colony formation assay in different cell lines: Neomycin resistant colonies were used to conclude the activity of CRs. Mouse CRs construct and control plasmids constructs were electroporated in different cell lines followed by drug selection till no colonies were left in negative control. X axis represents different plasmid constructs and Y axis total number of colonies formed, normalized to 100. The data suggests that CRs work as repressors. CR1, CR2, and CR6 show strong repression activity as compared with CR3.