Skip to main content
. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2017 Mar 10;1861(11 Pt B):3009–3015. doi: 10.1016/j.bbagen.2017.03.003

Figure 1.

Figure 1

Design and growth rate of E. coli MEOV1. A. Schematic representation of MEOV1 cells. A copy of the C. crescentus HisRS and tRNAHis expression cassette (shown in the blue box as Cc hisST) was introduced into the E. coli TOP10 chromosome to give MEOV0.1. The high-copy plasmid pUC18 harboring a copy of the C. crescentus HisRS and tRNAHis expression cassette was transformed into MEOV0.1 followed by deletion of the E. coli HisRS gene (hisS, shown in dark green box) from the chromosome and mutagenesis of anticodon (GUG to CUA) of the E. coli tRNAHis gene (hisR, show in in light green box). B. Growth curves of E. coli TOP10 (black circles) and MEOV1 (red squares).