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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2017 Mar 10;1861(11 Pt B):3009–3015. doi: 10.1016/j.bbagen.2017.03.003

Table 1.

Suppression efficiency of amber (UAG), ochre (UAA), and opal (UGA) codons at position 2 of sfGFP by the E. coli HisRS and tRNAHis with the corresponding anticodon sequence.

tRNAHisXXX sfGFP2XXX Suppression efficiency (%)*
GUG CAC -
CUA UAG 44.3 ± 11
UUA UAA 33.1 ± 2.5
UCA UGA 5.9 ± 2.6
*

Suppression efficiency is relative to wild-type sfGFP. The percentage values are the result of three independent assays, and the standard deviation is shown.