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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Eur J Cell Biol. 2017 Mar 28;96(5):407–417. doi: 10.1016/j.ejcb.2017.03.007

Fig. 6. PAM/Ab complexes and MPR/Ab complexes are internalized together.

Fig. 6

After 10 min of uptake, MPR/Ab complexes had begun to collect in the TGN (A) and co-localized with PAM/Ab complexes in endosomes (A, right panel). After 20 min of PAM/Ab and MPR/Ab uptake, co-localization was observed in the TGN and in endosomes (B). Scale bars = 10 μm. The overlap in endosomes was partial, but more than 90% of the fluorescently labeled endosomes contained both cargos after 10 and 20 min (mean ± SE for 3 separate experiments). (C). When examined at the EM level using gold-tagged antibodies, multivesicular bodies representing 20 min of uptake of PAM and MPR antibodies generally contained both cargos; arrowheads mark ILVs that contained both cargoes (D). Arrow at left shows MPR/Ab complex in a tubule directed towards the Golgi complex (G). Scale bars = 200 nm.