The serum antibody levels in NOD males (A-D) and females (E-H) on PicoLab diet. IgG2a (A), IgG2b (B), IgM (C), and IgG1 (D) levels in the sera of NOD males were measured at dilutions of 1:50, 1:50, 1:500, and 1:5000 (v/v), respectively, by ELISA. IgG2a (E), IgG2b (F), IgM (G), and IgG1 (H) levels in the sera of NOD females were determined at dilutions of 1:50, 1:50, 1:500, and 1:5000 (v/v), respectively. The sera were obtained when the mice were euthanized. *, p<0.05, and **, p<0.01. Each of the serum antibody concentration was calculated as the percent control. VHM = NOD males dosed with vehicle (VH, N = 10); VHF = NOD females dosed with VH (N = 8); GEM = NOD males dosed with GEN (N = 10); and GEF = NOD females dosed with GEN (N = 9).