Table 3. The cytokine/chemokine levels measured by Multiplex Mouse Cytokine/chemokine Magnetic Bead Panel Kit 96 Well Plate Assay in NOD male and female sera.
| G-CSF | EOTAXIN | GM-CSF | IFN-γ | IL-1α | IL-1β | IL-2 | IL-4 | |
|---|---|---|---|---|---|---|---|---|
|
|
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| VHF | 131.95±28.09 | 288.22±71.23 | 10.17±4.05 | 5.59±2.39 | 76.99±22.33 | 5.02±1.46 | 10.63±4.94 | n.d. |
| GEF | 242.24±26.27* | 437.94±66.63 | 22.08±3.79* | 11.58±2.24 | 168.89±24.32* | 10.18±1.37* | 19.68±4.62 | n.d. |
| VHM | 163.15±32.98 | 676.00±51.06 | 24.58±5.88 | 8.17±2.00 | 239.96±27.22 | 14.70±2.25 | n.d. | n.d. |
| GEM | 195.36±36.88 | 565.61±57.09 | 6.00±6.57* | 1.87±2.20* | 222.21±30.43 | 11.40±2.52 | n.d. | n.d. |
|
|
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| IL-3 | IL-5 | IL-6 | IL-7 | IL-9 | IL-10 | IL-12p40 | IL-12p70 | |
|
|
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| VHF | n.d. | 8.23±2.32 | 4.14±2.44 | 16.90±5.30 | 243.98±64.56 | 3.41±0.41 | n.d. | 3.72±0.82 |
| GEF | n.d. | 9.34±2.17 | 8.00±2.29 | 7.51±8.39 | 330.15±60.39 | 4.15±0.39 | n.d. | 4.27±0.76 |
| VHM | n.d. | 7.01±0.61 | 13.08±3.88 | n.d. | 356.53±59.57 | 6.07±1.13 | n.d. | 4.67±0.87 |
| GEM | n.d. | 4.46±0.68* | 3.17±4.33 | n.d. | 176.75±66.60 | 3.32±1.27** | n.d. | 2.92±0.98 |
|
|
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| LIF | IL-13 | LIX | IL-15 | IL-17 | IP-10 | KC | MCP-1 | |
|
|
||||||||
| VHF | n.d. | 10.75±3.07 | 914.39±330.69 | 352.19±98.54 | n.d. | 227.06±36.51 | 67.57±17.99 | 18.64±3.62 |
| GEF | n.d. | 19.36±2.87 | 2014.00±309.33* | 51.96±92.17 | n.d. | 239.84±34.15 | 101.03±16.83 | 24.80±3.39 |
| VHM | n.d. | 22.59±4.83 | 1722.36±223.59 | 66.90±61.97 | n.d. | 220.87±18.00 | 108.84±18.35 | 26.43±2.65 |
| GEM | n.d. | 12.09±0.81 | 1107.63±249.98 | 198.54±69.28 | n.d. | 189.77±20.13 | 80.87±20.51 | 19.08±2.97* |
|
|
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| MIP-1α | MIP-1β | M-CSF | MIP-2 | MIG | RANTES | VEGF | TNF-α | |
|
|
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| VHF | 97.75±35.84 | 22.62±6.23 | n.d. | 79.04±10.40 | 224.64±44.65 | 7.52±0.63 | 3.96±1.36 | 2.88±0.42 |
| GEF | 165.01±33.53 | 41.03±5.84 | n.d. | 125.41±9.73* | 147.07±41.76 | 8.06±0.59 | 6.34±1.22 | 4.37±0.40* |
| VHM | 131.70±22.89 | 36.03±6.29 | 5.65±0.85 | 129.76±5.05 | 263.06±30.66 | 11.41±1.28 | 4.79±0.61 | 5.09±0.38 |
| GEM | 64.72±25.59 | 20.21±7.04 | 7.29±0.95 | 116.15±5.65 | 265.15±34.28 | 10.10±1.43 | 3.74±0.69 | 4.09±0.42 |
Note: All values represent mean ± S.E. (pg/ml). IL = interleukin, IP-10 = interferon γ-induced protein 10, G-CSF = granulocyte-colony stimulating factor, GM-CSF = granulocyte macrophage colony-stimulating factor, LIX = C-X-C motif chemokine 5, KC = keratinocyte chemoattractant, MCP-1 = monocyte chemoattractant protein-1, MIP-1α = macrophage inflammatory protein 1-α, MIP-1 β = macrophage inflammatory protein 1- β, MIG = C-X-C motif chemokine 9, RANTES = C-C motif ligand 5, VEGF = vascular endothelial growth factor, TNF-α = tumor necrosis factor-α, LIF = leukemia inhibitory factor, M-CSF = macrophage colony-stimulating factor, n.d. = not detected, VHM = NOD males dosed with vehicle (VH, N = 10); VHF = NOD females dosed with VH (N = 8); GEM = NOD males dosed with genistein (GEN, N = 10); and GEF = NOD females dosed with GEN (N = 9).
P<0.05,
P<0.01.