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. Author manuscript; available in PMC: 2018 Nov 1.
Published in final edited form as: Cell Signal. 2017 Aug 3;39:74–83. doi: 10.1016/j.cellsig.2017.08.001

Figure 2. CDK1 phosphorylates PBK in vitro.

Figure 2

A, in vitro kinase assays with purified CDK1/cyclin B complex and recombinant GST-PBK. RO3306 (5 μM) was used to inhibit CDK1 kinase activity. B, Conservation of PBK’s mitotic phosphorylation sites. C, in vitro kinase assays with purified CDK1/cyclin B complex to phosphorylate recombinant GST-PBK or GST-PBK-4A. D, In vitro kinase assays were done as in (A) except anti-phospho-PBK T9, T24, S32 and S59 antibodies were used.