Table 3. Spiking recovery* test of calibration serum 742 with 1/250 dilution and with the lowest antibody concentration serum in the middle of 5 sera tested in this assay for each anti-PnPS (acceptable range: 100% ± 40%).
| Spike | PnPS | ||||||
|---|---|---|---|---|---|---|---|
| 4 | 6B | 9V | 14 | 18C | 19F | 23F | |
| Low spike (1/40) | 74.1 | 154.5 | 134.8 | 106.2 | 76.9 | 165.6 | 108.6 |
| Medium spike (1/80) | 70.5 | 126.9 | 133.4 | 103.5 | 70.9 | 139.8 | 120.3 |
| High spike (1/160) | 73.7 | 138.8 | 101.1 | 117.4 | 73.4 | 143.1 | 95.9 |
PnPS = pneumococcal polysaccharide.
*Recovery (%) = (observed concentration/expected concentration) × 100.