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. 2017 Sep 6;10:284. doi: 10.3389/fnmol.2017.00284

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Copyright © 2017 Deuis, Dvorakova and Vetter.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Temperature preference assays. (A) Two-temperature choice assay. Rodents are allowed to freely move between a reference plate (neutral temperature) and test plate. The time spent on the test plate relative to the reference plate is measured over a set period of time. (B) Continuous temperature gradient assay. Rodents are allowed to freely move along a liner or circular surface with a temperature gradient. The time taken to settle in a temperature zone and/or the temperature of the chosen zone is recorded.