Table 1.

Bacterial strains and plasmids used in this study.

Strains or plasmids	Relevant characteristicsa	Source or references
STRAIN
Escherichia coli JM109	recA1, endA1, gyrA96, thi, hsdR17, supE44,relA1, λ−, Δ(lac-proAB), [F′, traD36 proAB, lacIqZΔM15]	Kanchanarach et al., 2010
PLASMIDS
pEASYTM-T1	Apr, Kmr, f1 ori	TransGen Biotechb
PBBR1MCS-4c	Apr, ColE1 ori PBBR1MCS-4 carrying Padh	Yanisch-Perron et al., 1985; Kovach et al., 1995
PBBR-adhA	PBBR1MCS-4 carrying adhA	This study
PBBR-adhB	PBBR1MCS-4 carrying adhB	This study
PBBR-adhA-adhB	PBBR1MCS-4 carrying adhA and adhB	This study

^aAp^r, ampicillin resistance; Km^r, kanamycin resistance.

^bTransGen Biotech, Beijing, China.

^cA 367-bp fragment containing Padh, the adh promoter was amplified by PCR and cloned into PBBR1MCS-4, as described in Kovach et al. (1995) (Figure 1).