Table 2.
Primers | Sequence | Restriction site |
---|---|---|
adhA-P1 | CGCGGATCCATGACCCGCCCCGCCTCCGCCAAGA | BamH I |
adhA-P2 | TGCACTAGTTTAGGGGTTAATGCCAAGTGTCG | Spe I |
adhB-P1 | CGCACTAGTGATGATGATGAACAGGCTAAAAACTGC | Spe I |
adhB-P2 | AGCTCTAGATTACTGGGCTTCATCCACACCAGCA | Xba I |
Padh-P1 | CGCGGATCC ATCCACCACAGCCTGCGTGCACCAGA | BamH I |
Padh-P2 | AGCACTAGTGCGTTCATGTCCTCGACTATTATATA | Spe I |
The underlined sequences represent the restriction sites.