Table 4.
Reactions and enzymes in the metabolic pathway of Acetic acid in A. pasteurianus JST-S.
| NO. | Enzyme | Metabolic reactions |
|---|---|---|
| 1 | Glucokinase | ATP + Glc = ADP + G6P |
| 2 | Glucose-phosphate-isomerase | G6P = F6P |
| 3 | Glucose-6-phosphate dehydrogenase | G6P + 2 × NADP+ = 2 × NADPH + Ru5P + CO2 |
| 4 | Transketolase/Transaldolase | 3 × Ru5P = 2 × F6P + G3P |
| 5 | Phosphofructokinase /Aldolase/ Triose-phosphate-isomerase | ATP + F6P = ADP + 2 × G3P |
| 6 | Enolase /Phosphoglycerate-kinase / Phosphoglyceromutase /Glyceraldehydes -phosphate-dehydrogenase | G3P + NAD+ + Pi + ADP = PEP + NADH + ATP + H2O + H+ |
| 7 | Pyruvate-kinase | ADP + PEP = ATP + Pyr |
| 8 | Phosphoenolpyruvate-carboxykinase | CO2+ PEP + ADP = OAA + ATP |
| 9 | Pyruvate dehydrogenase | Pyr + CoA + NAD+ = Ac-CoA + CO2+ NADH |
| 10 | Pyruvate decarboxylase | Pyr = AC.H. + CO2 |
| 11 | Lactate dehydrogenase | Pyr + NADH + H+ = Lac + NAD+ |
| 12 | Pyruvate carboxylase | ATP + Pyr + = ADP + Pi + OAA |
| 13 | Phosphotransacetylase /acetate kinase | Ac-CoA + Pi + ADP = CoA + Ace + ATP |
| 14 | Alcohol dehydrogenase | EtOH + NAD+ = AC.H. + NADH + H+ |
| 15 | Acetaldehyde dehydrogenase | AC.H. + NAD+ = Ace + NADH + H+ |
| 16 | Citrate synthase | Ac-CoA + H2O + OAA = Cit + CoA |
| 17 | Aconitase | Cit = Isoc |
| 18 | Isocitrate dehydrogenase | Isoc + NADP+ = α-Ket + NADPH + CO2 |
| 19 | Oxoglutarate dehydrogenase complex | α-Ket + NAD+ = Suc + NADH + H+ + CO2 |
| 20 | Fumarate reductase | Suc + FAD+ = Fum + FADH |
| 21 | Fumarate hydratase | Fum + H2O = Mal |
| 22 | Malate dehydrogenase | Mal + NAD+ = OAA + NADH + H+ |
Glc, glucose; G6P, glucose-6-phosphate; F6P, fructose-6-phosphate; Ru5P, ribulose 5-phosphate; G3P, glyceraldehydes-3-phosphate; PEP, phosphoenolpyruvate; Pyr, pyruvate; AC.H., Acetaldehyde; OAA, oxaloacetate; Acetyl-CoA, acetyl coenzyme A; CoA, Coenzyme A; Lac, Lactate; Ace, Acetate; EtOH, ethanol; Cit, citrate; Isoc, Isocitrate; α-Ket, α-ketoglutaric acid; Suc, succinic acid; Fum, fumarate; Mal, Malate.