Table 3.

Antibacterial activity of the extracts from endophytic actinomycetes in two culture media (SG and R5A) and two temperatures (28°C and 36°C) against Clinical pathogens.

Strain/Genera	Antimicrobial activity (inhibition zone in mm)
	Staphylococcus aureus (Figure S1)				Meticilin-resistant S. aureus (Figure S1)				Escherichia coli (Figure S2)
	Medium SG		Medium R5A		Medium SG		Medium R5A		Medium SG		Medium R5A
	28°C	36°C	28°C	36°C	28°C	36°C	28°C	36°C	28°C	36°C	28°C	36°C
Actinomadura sp. LGMB466	9.75 ± 0.5	11.25 ± 0.5	11.25 ± 0.5	9 ± 0.8	9.5 ± 0.58	9.25 ± 0.5	9.5 ± 0.6	9.5 ± 0.6	12, 0	12.75 ± 0.5	12.75 ± 0.5	10.75 ± 0.5
Actinomadura sp. LGMB487	9.75 ± 0.5	11.75 ± 1	11.75 ± 1	9.5 ± 0.6	10.5 ± 1	9.5 ± 0.58	9.5 ± 0.6	9 ± 0.8	11.0	11.5 ± 0.6	12.25 ± 1	11.0
Aeromicrobium ponti LGMB491	20.5 ± 0.6	22 ± 1.3	19.25 ± 1.3	15.75 ± 1.7	24.2 ± 2.06	19.8 ± 0.5	11.5 ± 1.3	17.25 ± 1.7	11.5 ± 1	12 ± 0.8	10.5 ± 0.6	10.25 ± 0.5
Microbacterium sp. LGMB471	11.25 ± 1	9.5 ± 1	9.5 ± 1	11 ± 1.2	11.25 ± 0.96	8.25 ± 1.3	11.75 ± 1.5	9.25 ± 0.5	11.25 ± 0.5	12.25 ± 0.5	13 ± 0.8	11.0
Microbispora sp. LGMB461	8.75 ± 1.5	9.5 ± 1.0	9.5 ± 1.0	9.25 ± 1.5	9.75 ± 1.7	8.5 ± 1	10.75 ± 0.5	8.75 ± 0.5	12.75 ± 0.5	9.5 ± 0.6	12.25 ± 1	12.5 ± 0.6
Microbispora sp. LGMB465	10 ± 0.8	9.25 ± 0.5	9.25 ± 0.5	9.5 ± 0.6	9.5 ± 1	9.75 ± 0.96	8.0	11.5 ± 0.6	13.25 ± 1	11.5 ± 0.6	8.0	10.5 ± 0.6
Micrococcus sp. LGMB485	10.25 ± 0.5	12.5 ± 1.7	12.5 ± 1.7	10.5 ± 1.3	10 ± 0.82	11.2 ± 1.26	10 ± 1.4	8.75 ± 0.5	10.5 ± 0.6	10.75 ± 0.5	12.75 ± 0.5	10 ± 0.8
Sphaerisporangium sp. LGMB482	10.5 ± 1.3	10.25 ± 1.7	10.25 ± 1.7	11, 0	9.5 ± 0.58	10.5 ± 1.3	9.5 ± 0.6	9.5 ± 0.6	11 ± 0.8	9.75 ± 1	12 ± 0.8	11 ± 0.8
S. thermocarboxydus LGMB483	10.75 ± 1.5	10.0	10.0	11.25 ± 1.0	11.2 ± 0.96	11.2 ± 0.96	8.75 ± 1.5	8.5 ± 1	11.5 ± 1.3	11 ± 0.8	11, 0	8.25 ± 0.5
Williamsia serinedens LGMB479	12 ± 1.8	9.75 ± 2.2	9.75 ± 2.2	10.25 ± 0.5	12 ± 1.83	10.2 ± 0.96	10.0	10.25 ± 0.96	11.5 ± 0.6	9.5 ± 0.6	13.5 ± 1.3	12.25 ± 1
Strain/Genera	Antimicrobial activity (inhibition zone in mm)
	P.aeruginosa (Figure S3)				A.baumanni (Figure S4)				C. albicans (Figure S5)
	Medium SG		Medium R5A		Medium SG		Medium R5A		Medium SG		Medium R5A
	28°C	36°C	28°C	36°C	28°C	36°C	28°C	36°C	28°C	36°C	28°C	36°C
Actinomadura sp. LGMB466	9.25 ± 1	10 ± 0.8	9 ± 0.8	8.5 ± 1.3	10.5 ± 0.6	10 ± 0.8	10.5 ± 0.6	9.75 ± 1.3	9.75 ± 1	10.25 ± 0.5	9.75 ± 1.3	10 ± 0.8
Actinomadura sp. LGMB487	10.25 ± 1	7.75 ± 1	9.25 ± 0.5	7.75 ± 1	9.25 ± 0.9	10 ± 0.8	9.25 ± 1.9	9.5 ± 0.6	8 ± 0.8	7.5 ± 0.6	10.75 ± 1.9	7.75 ± 0.5
Aeromicrobium ponti LGMB491	10.5 ± 1	10.5 ± 2.9	9 ± 1.2	13 ± 1.8	12.5 ± 1	10.5 ± 2.1	9 ± 0.8	11.75 ± 1	10.25 ± 1.3	8.5 ± 1	8 ± 1.4	10.00
Microbacterium sp. LGMB471	9.0	10 ± 0.8	10.25 ± 1	10 ± 1.4	9.5 ± 0.6	10.5 ± 1.7	9.75 ± 0.5	9.25 ± 1	13.5 ± 0.6	10.75 ± 1.3	11.00	9.75 ± 0.5
Microbispora sp. LGMB461	8.25 ± 0.5	9.0	11.25 ± 1.5	9.0	8.5 ± 1	9.75 ± 0.5	9.00	10.75 ± 1	8.75 ± 1.5	9.25 ± 1	8.5 ± 0.6	8.75 ± 0.5
Microbispora sp. LGMB465	11 ± 1.4	10 ± 0.8	9.5 ± 0.6	10.5 ± 1	11.00	9 ± 0.8	10.25 ± 1	11.00	10.75 ± 0.5	10.25 ± 1	9.75 ± 0.5	11 ± 2
Micrococcus sp. LGMB485	9.0	10 ± 1.2	8.75 ± 1	9.0	10 ± 0.8	9.5 ± 1	9.25 ± 1.5	11.25 ± 0.5	11.25 ± 1	10.25 ± 0.5	7.5 ± 0.6	9.5 ± 1
Sphaerisporangium sp. LGMB482	10.5 ± 1.7	8.75 ± 0.5	10.25 ± 1	9.75 ± 1	11.25 ± 0.9	9.75 ± 1.7	10 ± 0.8	10 ± 0.8	11.75 ± 1.5	10.75 ± 1	13 ± 0.8	11.75 ± 1.5
S. thermocarboxydus LGMB483	11 ± 1.4	11.25 ± 1.5	7.5 ± 0.6	8.5 ± 0.6	9.75 ± 0.5	11 ± 1.4	7.75 ± 1	8.75 ± 0.9	10 ± 1.4	8.75 ± 0.5	7.75 ± 0.5	7.5 ± 0.6
Williamsia serinedens LGMB479	10.75 ± 2.1	10 ± 1.2	10 ± 1.2	9.5 ± 0.6	12.5 ± 1.9	9.5 ± 0.6	10.5 ± 1	10.75 ± 1.3	11 ± 2	11 ± 2	12.25 ± 1.7	10.25 ± 1.5
Strain/Genera	Antimicrobial activity (inhibition zone in mm)
	E. Cloacae producer of VIM (Figure S6)				S. malthophilia (Figure S7)				Klebissiella pneumoniae producer of KPC (Figure S8)
	Medium SG		Medium R5A		Medium SG		Medium R5A		Medium SG		Medium R5A
	28°C	36°C	28°C	36°C	28°C	36°C	28°C	36°C	28°C	36°C	28°C	36°C
Actinomadura sp. LGMB466	9.75 ± 0.5	10 ± 0.8	10.25 ± 0.5	10.5 ± 0.6	10.5 ± 1.3	11.75 ± 1	9.5 ± 0.6	9.25 ± 0.5	10.75 ± 0.5	10.5 ± 0.6	11.25 ± 1	10.25 ± 0.5
Actinomadura sp. LGMB487	11.25 ± 0.5	10.5 ± 0.6	10.75 ± 0.5	9.5 ± 0.6	11.25 ± 1	9 ± 0.8	11 ± 0.8	9.00	9.75 ± 1	8.25 ± 0.5	10.25 ± 0.5	8.5 ± 0.6
Aeromicrobiumponti LGMB491	10.75 ± 1	10.5 ± 1.3	12.25 ± 1.7	12.25 ± 1	13.5 ± 0.6	13.25 ± 1.5	9.00	12.25 ± 0.5	10 ± 0.8	10.75 ± 1	8.75 ± 0.5	10.5 ± 1
Microbacterium sp. LGMB471	11.25 ± 0.5	10.00	10.00	10.25 ± 0.5	12.5 ± 0.6	9.75 ± 0.5	10.75 ± 0.5	10.5 ± 0.6	11.75 ± 0.5	9.75 ± 1	11.25 ± 0.5	10 ± 0.8
Microbispora sp. LGMB461	8.75 ± 1	10.25 ± 1	10.5 ± 1.3	10.75 ± 1	10.25 ± 1.3	10.5 ± 1.3	11 ± 0.8	10.25 ± 0.5	11 ± 1.8	10.25 ± 0.5	9.25 ± 0.5	11.00
Microbispora sp. LGMB465	10.25 ± 1	9.5 ± 0.6	10.75 ± 0.5	10.25 ± 1.3	9.25 ± 1	9.75 ± 0.5	9.5 ± 1	9.5 ± 0.6	9.75 ± 0.9	11.5 ± 0.6	10 ± 0.8	9.25 ± 0.5
Micrococcus sp. LGMB485	12.25 ± 1.3	11.5 ± 0.6	10.75 ± 1	10 ± 1.4	11.75 ± 0.5	10.00	10.5 ± 0.6	10.5 ± 0.6	9.5 ± 0.6	11.25 ± 0.5	8.5 ± 0.6	10.00
Sphaerisporangium sp. LGMB482	11.5 ± 0.6	10.5 ± 0.6	10.75 ± 0.5	9.00	11.5 ± 1	10.75 ± 0.5	11.00	10.25 ± 1	12.75 ± 1.2	10.75 ± 0.5	12.00	9.75 ± 0.5
S.thermocarboxydus LGMB483	11.5 ± 0.6	12.00	10.25 ± 0.5	8.5 ± 0.6	11.5 ± 0.6	11.75 ± 1	9.75 ± 1	10.75 ± 1	11.00	12 ± 0.8	9.75 ± 0.9	8.5 ± 0.6
Williamsia serinedens LGMB479	10.75 ± 0.5	10.5 ± 0.6	11.25 ± 0.5	9.25 ± 0.5	12.00	12.25 ± 1	12 ± 1.2	11.25 ± 1	12.75 ± 1.2	12.25 ± 1	12 ± 0.8	12.25 ± 1

Mean (±SD).