Figure 5.

The fast and slow network responses to 10, 50, and 500 nM exendin-4 or exendin-4 + LPS. Data in (A,D) and (C,F) were obtained from the same dish in two different experiments performed 3 DIV apart. (A–C) NormEXC plot obtained with 10, 50, and 500 nM exendin-4 (EX-4) preconditioning, and 3 μg/ml LPS. The continuous line is the fixed threshold. Note that the transient and fast increase of normEXC became progressively higher with increasing EX-4 concentration. (D–F) CumBD plots of the experiments shown in (A–C). Control, thick line; EX-4, asterisk; rightward triangles are for the late LPS effects. The vertical arrow indicates the fixed threshold. Note that preconditioning with EX-4 at each concentration tested, effectively prevented the development of the late excitatory responses to LPS.