Table 1.

Purification of recombinant mesaconate CoA-transferase (Mct) and mesaconyl-CoA hydratase (Mch) from H. hispanica.

Enzyme	Purification step	Total protein (mg)	Total activity (U)	Specific activity (U mg-1)	Enrichment factor	Yield (%)
Mct	Cell extract	66.8	7.05	0.105	1	100
	Nickel column	0.94	6.6	7.02	67	93
	Gel filtration	0.13	2.19	16.9	161	31
Mch	Cell extract	290	107	0.368	1	100
	Nickel column	0.80	96.1	120	327	90
	Gel filtration	0.070	11.01	157	427	10.3

Activity was tested with 1 mM succinyl-CoA and 10 mM mesaconate (Mct) and with 0.5 mM β-methylmalyl-CoA (Mch).