Table 3.
Influence of divalent cations on activity of recombinant Mct and Mch from H. hispanica.
| Substratea | Mct, % | Mch, % |
|---|---|---|
| No additionb | 100b | 100b |
| EDTA | 109 ± 1 | 100 ± 5 |
| MgCl2 | 93 ± 1 | 89 ± 5 |
| MnCl2 | 76 ± 1.5 | 78 ± 10 |
| NiCl2 | 95 ± 0.5 | 66 ± 8 |
| CoCl2 | 90 ± 0.2 | 60 ± 8 |
| CaCl2 | 95 ± 0.1 | 82 ± 7 |
The values shown are mean values ± SD of results from typically three independent measurements. aActivity was tested with 1 mM succinyl-CoA and 10 mM mesaconate (Mct) or 0.5 mM β-methylmalyl-CoA (Mch). The concentration of divalent cations was 5 mM, EDTA 2 mM. b100% activity corresponds to 15.8 ± 0.1 (Mct) and 185 ± 14 (Mch) U mg-1 of protein.