Table 4.
MU distribution among mosquitoes developmental stages, cuticles, and breeding waters.
| Nature of the samples | Mosquitoes developmental stages | Distribution of MU targets | Pool positive/pool tested | Presence of MU | |
|---|---|---|---|---|---|
| Mean Cts (IS2404) | Mean Cts (KR-B) | ||||
| Mosquito∗ | Eggs | 19 ± 1.79 | 21 ± 2.22 | 4/4 | Yes |
| L1 | 27.67 ± 2.66 | 31.59 ± 3.15 | 4/4 | Yes | |
| L2 | 29.92 ± 2.58 | 33.06 ± 2.98 | 4/4 | Yes | |
| L3 | 31.36 ± 2.98 | 34.33 ± 3.34 | 4/4 | Yes | |
| L4 | 31.38 ± 2.20 | 35.03 ± 1.17 | 3/4 | Yes | |
| Pupae | NoCt | NoCt | 0/4 | No | |
| Adults | 37.89 | NoCt | 0/4 | No | |
|
| |||||
| Mosquito cuticles∗ | Eggs | NA | NA | NA | NA |
| L1 | 30.72 ± 1.78 | 36.51 ± 2.09 | 4/4 | Yes | |
| L2 | 34.25 ± 2.83 | 36.82 ± 1.65 | 3/4 | Yes | |
| L3 | 34.13 | 39.53 | 1/4 | Yes | |
| L4 | NoCt | NoCt | 0/4 | No | |
| Pupae | 38 | NoCt | 0/4 | No | |
| Adults | NA | NA | NA | NA | |
|
| |||||
| Mosquito breeding waters∗ | Eggs | 18.43 ± 2.03 | 21.49 ± 1.63 | 4/4 | Yes |
| L1 | 23.04 ± 3.19 | 30.61 ± 2.80 | 4/4 | Yes | |
| L2 | 22.71 ± 2.59 | 31.88 ± 2.60 | 4/4 | Yes | |
| L3 | 28.4 ± 2.86 | 33.53 ± 3.00 | 4/4 | Yes | |
| L4 | 32.00 ± 2.64 | 35.94 ± 1.04 | 3/4 | Yes | |
| Pupae | 33.65 | 35.47 | 1/4 | Yes | |
| Adults | NA | NA | NA | NA | |
L1, 2, 3, and 4 correspond to first, second, third, and fourth instars larvae, respectively. Yes or no corresponds to the presence or the absence of the bacteria in analyzed samples. NA stands for not applicable. ∗The bacterial loads did not vary significantly among the developmental stages (p < 0.05).