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In vitro human blood-clot lysis assay. (a) Recombinant PI239 dissolved human blood clot. (b) Percentage of blood-clot lysis. PBS: 1x Phosphate Buffered Saline; −CK: elution from noninfiltrated leaf (negative control); WT: pBYR-WT-PI239; OP: pBYR-LPH-OP-PI239. LK: standard lumbrokinase (18,000 u/mg). Values represent the means ± SE. Different letters indicate significant differences (p ≤ 0.01; n = 3; one-way ANOVA test).
