Figure 10.

Persistent loss of oligodendrocytic Cx47 in mouse whole-brain protein. C57Bl/6 mice were mock– or MHV-A59–infected, and proteins were extracted from brain at day 5 p.i. (acute phase) and at day 30 p.i. (chronic phase). Whole-brain proteins were probed for Cx47 (detected at nearly 47 kDa) and internal control, γ-actin (detected at nearly 42 kDa). Oligodendrocytic Cx47 was reduced in brain in the acute stage of inflammation, at day 5 p.i. (A). There was ∼32.78% depletion reduction (normalized with γ-actin) of Cx47 in the whole-brain protein (B). The mean ± S.D. (error bars) incidences from three different animals are shown (***, p < 0.001). At the peak of demyelination at day 30 p.i., Cx47 was not replenished back to its normal level (C). Both of the Cx47-immunoprobed blots showed a nonspecific signal at about 51 kDa. A persistent ∼35.83% reduction in Cx47 expression signal was observed upon normalization with internal control γ-actin (D; ****, p < 0.0001). Error bars, S.D.