Figure 8.

Interaction between viral particles and β-tubulin. To understand whether reduced Cx43–β-tubulin interaction was due to interaction between viral particle and MTs, co-IP was performed using polyclonal anti-β-tubulin antibody. The immunoprecipitated samples were probed with monoclonal anti-N antibody. Samples were probed for γ-actin, as an internal control. Viral N protein was selectively detectable near 50 kDa, in the MHV-A59–infected sample only, whereas γ-actin expression was similar between mock- and virus-infected cells. MHV-A59–infected cells also showed that viral N-protein interacted with β-tubulin. The beads that were used in preclearing showed absence of nonspecific signal upon probing with anti-Cx43.