Table 1.
Primary antibodies used for immunofluorescence, co-IP, and Western blotting
Secondary antibodies were as described in the text.
| Antibodies | Dilution/Amount used | Source |
|---|---|---|
| Polyclonal anti Cx43 antibody (rabbit) | Immunofluorescence: 1:200 | C6219, Sigma |
| Western blotting: 1:1000 | ||
| Polyclonal anti β-tubulin antibody (rabbit) | Immunofluorescence: 1:100 | BB-AB0119, Biobharati LifeSciences Pvt. Ltd. (Kolkata, India) |
| Co-IP: 3 μg | ||
| Monoclonal anti β-tubulin antibody (mouse) | Immunofluorescence: 1:200 | Clone TUB 2.1, T4026, Sigma |
| Co-IP: 2 μg | ||
| Monoclonal anti Nucleocapsid (N) antibody (mouse) | Immunofluorescence: 1:50 | Kindly provided by Dr. Julian Leibowitz |
| Western blotting: 1:100 | ||
| Polyclonal anti-Cx47 antibody (rabbit) | Immunofluorescence: 1:50 | Invitrogen (catalog number: 36-4700) |
| Western blotting: 1:1000 | ||
| Anti-PLP antibody (rat) | Immunofluorescence: 1:1 | Kindly provided by Judith B. Grinspan (Children's Hospital of Philadelphia, Philadelphia, PA) |