Figure 1.

UVA induces expression of autophagy and lysosomal genes. A, schematic of RNA-Seq analysis of normal human epidermal keratinocytes (NHEK) at 6 h post-sham or -UVA (20 J/cm²) radiation. Gene expression and pathway analysis were performed using the Tuxedo pipeline and DAVID. B, volcano plot representing genes significantly differentially expressed between sham- and UVA-irradiated NHEKs as determined by RNA-Seq. p < 0.05. C, pathway analysis of the significantly up-regulated genes by RNA-Seq identified highly enriched pathways following UVA irradiation in NHEKs. D, real-time PCR validation of RNA-Seq results in NHEKs treated with UVA. *, p < 0.05; **, p < 0.01 (Student's t test). Error bars, S.D.