p62 is up-regulated by UVA independent of autophagy.
A, p62 protein levels in NHEKs 0, 1.5, and 6 h after UVA radiation (10 J/cm2). B, p62 protein levels 6 h after irradiation with 0, 5, 10, or 15 J/cm2 UVA. C, immunohistochemical staining of p62 in mouse skin after UVA irradiation (30 J/cm2) every other day for a total of three treatments and collected at 72 h after the final UVA irradiation. D, p62 protein levels in the SCC cell line PAM212 at 0, 1.5, and 6 h after UVA irradiation. E, p62 protein levels in irradiated PAM212 cells treated with vehicle or the autophagic flux inhibitor bafilomycin A1. F, p62 protein levels in PAM212 cells transfected with shCon or shAtg7 after sham or UVA irradiation. G, p62 levels in iBMK cells transfected with shCon or shAtg5 after sham or UVA irradiation.