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. 2017 Jul 19;292(36):14786–14795. doi: 10.1074/jbc.M117.786160

Figure 6.

Figure 6.

p62 regulates COX-2 protein stability through the UBA domain. A, HeLa cells were transiently transfected with COX-2 and either empty vector or HA–p62. COX-2 stability was measured over time by treating with CHX for the time indicated. B, quantification of the stability of COX-2 protein in A. C, HA-tagged WT or mutant p62 lacking the UBA domain (dUBA) were transfected into HeLa cells. D, COX-2 stability was measured over time after treating with CHX for 0, 4, or 8 h and quantified. E, co-immunoprecipitation of p62 and COX-2 in PAM212 cells treated with the proteasome inhibitor MG132 (10 μm) for 6 h. F, transcription of COX-2 in HeLa cells transiently transfected with COX-2 and HA–p62 or HA–p62–dUBA 48 h after transcription. IB, immunoblotting. Error bars, S.D. *, p < 0.05; ***, p < 0.001.