p62 regulates PGE2 production but not proliferation or migration in vitro.
A, PGE2 production in PAM212 cells stably transfected with shCon or shp62. shp62 cells were transiently transfected with COX-2 (shp62+COX-2). n.s., not significant; *, p < 0.05; **, p < 0.01. B, PGE2 production in HeLa cells transiently transfected with EV, HA–p62, COX-2, and HA–p62–dUBA, as indicated. n.s., not significant; *, p < 0.05; **, p < 0.01. C, proliferation of PAM212 cells stably transfected with shCon or shp62 measured over 3 days using an MTS proliferation assay. D, migration of WT, shCon, and shp62 PAM212 cells measured using a Transwell migration assay in serum-free medium. Error bars, S.D.