p62 regulates tumor growth, metastasis, and immune infiltration in vivo.
A, shCon and shp62 PAM212 cells were injected into syngeneic BALB/c mice, and tumor growth was measured over 10 weeks. n = 6 mice/group. *, p < 0.05, Student's t test, significant difference as compared with the shCon group. B, metastasis to the lungs of mice injected with shCon and shp62 PAM212 cells was measured using H&E staining. n = 6 mice/group. *, p < 0.05, Student's t test, significant difference. C, H&E staining of tumors from mice injected with shCon and shp62 PAM212 cells. Black arrow, immune cell infiltration. Gray arrow, differentiated keratinocytes. Magnification was ×20; scale bar, 100 μm. Inset, ×40 magnification; scale bar, 50 μm. D and E, IHC staining for MPO (D) and K10 (E) in tumors from mice injected with shCon and shp62 PAM212 cells. Magnification was ×20; scale bar, 100 μm. Inset, ×40 magnification; scale bar, 50 μm; error bars, S.D.