Figure 1.
CTRP6 is broadly expressed in human tissues and up-regulated in obese humans. A, real-time PCR analysis of human CTRP6 expression in adult tissues using the TissueScanTM array (OriGene). Expression level of CTRP6 in each tissue was normalized to GAPDH (provided by the manufacturer). Expression in each tissue represents the average of pooled human samples to minimize individual variation. B–E, real-time PCR analysis of CTRP6 in omental and subcutaneous adipose tissue of human abdominal surgery subjects. Expression of CTRP6 in both fat depots is positively correlated with BMI (B and C). Expression levels of CTRP6 in both fat depots are significantly higher in obese individuals with or without type 2 diabetes relative to lean controls (D and E; n = 7–8/group). Expression levels were normalized to β-actin in each sample. **, p < 0.01. Error bars, S.E.