Figure 5.

The decrease in Snf2 levels over time in batch cultures of WT yeast correlates with enhanced splicing of PTC7 RNA. A, steady-state levels of Snf2 protein in WT cells corresponding to samples from indicated time points were determined by immunoblot. Pgk1 (phosphoglycerate kinase 1) served as a loading control. B, expression and splicing of PTC7 in WT yeast cells corresponding to samples from A; PCR products represent the spliced and nonspliced forms, as indicated. C, quantification of splicing of PTC7 transcript (black line) in WT yeast cells corresponding to samples from B. Shown is the mean of three biological replicates. Error bars, S.D. D, the Snf2 protein is absent in snf2Δ cells corresponding to samples from the indicated time points as determined by immunoblot. Pgk1 served as a loading control. E, expression and splicing of PTC7 in snf2Δ yeast cells corresponding to samples from D; PCR products representing the spliced and nonspliced forms are indicated. F, quantification of splicing of PTC7 transcript (red line) in snf2Δ yeast cells corresponding to samples from E. Shown is the mean of three biological replicates. Error bars, S.D. (unpaired Student's t test between corresponding bars for snf2Δ and WT in C; *, p < 0.05; **, p < 0.005).