Figure 7.

RPG down-regulation and redistribution of spliceosomes result in increased PTC7 splicing. A, RT-qPCR measurement of selected intron-containing RPG transcripts between WT yeast treated with rapamycin and a vehicle control. Mean of three biological replicates (unpaired Student's t test, **p < 0.005). Error bars, S.D. B, expression and splicing of PTC7 in WT yeast treated with rapamycin and a vehicle control (DMSO). PCR products representing the spliced and nonspliced forms are indicated. C, quantification of three independent biological replicates of B (unpaired Student's t test; ***, p < 0.0005). Error bars, S.D.