Figure 8.

KD of LGR5 in LoVo cells resulted in loss of IQGAP1 interaction with Rac1/actin and decreased cell–cell adhesion. A, results of calcein-AM cell–cell adhesion assay. Error bars are S.E. (n = 3). *, p < 0.05 and **, p ≤ 0.01 versus controls. B, representative WB analysis results of soluble (S) and insoluble (I) E-cadherin in LoVo cells with vector control, KD of LGR5 (shLGR5-89), or KD of IQGAP1 (shIQ1) when extracted with the indicated concentrations of Nonidet P-40 (NP40). C, quantification of WB results of E-cadherin. Error bars are S.E. (n = 3). *, p < 0.05 compared with vector cells. D, WB results of IQGAP1, actin, and Rac1 in control and LoVo cells with KD of LGR5 or IQGAP1. E, WB of serine phosphorylation of IQGAP1 in LGR5 KD LoVo cells.