Figure 5.

The cGAS–STING–IRF3 pathway mediates PA-induced MST1 up-regulation and YAP inhibition. HAECs were transfected with scramble siRNA or cGAS or STING or IRF3 siRNA and then treated with PA for 24 h. Western blot analysis showed that knocking down cGAS (A) or STING or IRF3 (B) prevented PA-induced activation MST1 expression and YAP phosphorylation (n = 6 biological repeats). C, representative images of immunostaining showed that knocking down STING or IRF3 prevented PA-induced YAP cytoplasm retention (n = 6 biological repeats).