Figure 5.

Vgll4 is phosphorylated during unperturbed mitosis. A and B, HeLa cells were synchronized by a double thymidine block and release method. Cells were stained with antibodies against p-Vgll4 Ser-155/Thr-159 (labeled as p-Vgll4 S155) or β-tubulin or with DAPI. A low power (40× objective) lens was used to view various phases of the cells in a field (B). C, experiments were done similarly as in B with p-Vgll4 Ser-280 antibodies. White and yellow arrows (in panels B and C) mark the metaphase and interphase cells, respectively.