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. 2017 Jul 24;292(36):15028–15038. doi: 10.1074/jbc.M117.796284

Figure 8.

Figure 8.

Mitotic phosphorylation of Vgll4 affected YAP and β-catenin activity and regulated the expression of cell cycle regulators. A and B, HEK293T cells were transfected with various DNA plasmids as indicated. The immunoprecipitates (IP, with FLAG or Myc antibodies) were probed with the indicated antibodies. Total cell lysates before immunoprecipitation were also included (input). C, quantitative RT-PCR for CTGF in S2.013 cell lines expressing vector, wild-type Vgll4, or Vgll4-4A. *, p < 0.05 (t test). D, luciferase reporter assays in HEK293T cells. **, p < 0.01; ***, p < 0.001 (t test). E, total cell lysates from S2.013 cell lines expressing vector, wild-type Vgll4, or Vgll4-4A were probed with the indicated antibodies (YAP-related). F, quantitative RT-PCR for LEF1 in S2.013 cell lines expressing vector, wild-type Vgll4, or Vgll4-4A. *, p < 0.05 (t test). G, total cell lysates from S2.013 cell lines expressing vector, wild-type Vgll4, or Vgll4-4A were probed with the indicated antibodies (β-catenin-related). H, total cell lysates were harvested from S2.013 cell lines expressing vector, wild-type Vgll4, or Vgll4-4A and were subjected to Western-blotting analysis with various cell cycle regulators.