Figure 2.

p38γ and c-Jun cooperate to stimulate EGFR RNA/protein expression. A, IEC-6 cells with tetracycline-inducible p38γ expression (Tet-on) were cultured in the absence or presence of Tet for the indicated times (left and middle); alternatively, cells were infected for 24 h with adenovirus expressing p38γ (Ad-p38γ) or β-galactosidase (Ad-β-Gal) (right). Protein and RNA samples were analyzed by WB and qRT-PCR, respectively (bar graph, mean ± S.D. (error bars), n = 3). B and C, p38γ was stably depleted by shRNAs through lentiviral infection of the indicated K-Ras mutant cells, and the resultant cells were analyzed for protein levels by WB (left) and for mRNA expression by qRT-PCR (right, mean ± S.D., n = 3) (14). D, c-Jun was stably expressed, and engineered cells were assessed for protein expression by WB and for colony formation (mean ± S.D., n = 3).