Figure 4.

There is an increased complex formation of EGFR with PTPH1 and p38γ in K-Ras mutant cells. A, equal protein amounts from K-Ras WT and MT cell lysates were precipitated with a specific EGFR antibody, and the precipitates were analyzed by WB for PTPH1 and p38γ (top left, *, IP with control IgG). PTPH1 and p38γ proteins in EGFR precipitates from K-Ras WT cells were measured and normalized by those from K-Ras MT cells (right panel, mean ± S.D. (error bars), n = 3, from top left). Direct Western blots of the inputs are shown (bottom, left). B, the indicated constructs were transiently expressed in 293T cells, and Myc precipitates were analyzed by WB for EGFR binding to HA-PTPH1 and/or FLAG-p38γ. C, cells were transduced with the indicated adenovirus and EGFR precipitates were analyzed by WB after a 24-h incubation.