Skip to main content
. Author manuscript; available in PMC: 2018 Mar 17.
Published in final edited form as: ACS Chem Biol. 2017 Jan 30;12(3):586–600. doi: 10.1021/acschembio.6b01148

Figure 2.

Figure 2

Representative ROS/RES target profiling and delivery strategies. ROS target profiling can be broken down into (a) “capping” strategies, wherein free thiols are capped before labeling of modified Cys (in this example, Cys-SOH), and (b) “chemoselective” strategies that use a chemoselective reagent to “pick out” a specific modification, such as Cys-SOH and label it selectively. Combinations of these strategies can be used in “competition,” wherein simultaneous chemoselective reactions are run on the same sample. RES target profiling can similarly be broken down into (c) activity-based proteomic profiling (ABPP) which utilizes a stepwise competition approach or (d) “direct profiling,” in which cells/lysates are treated with a functionalized probe and directly detected by MS analysis. Delivery strategies for ROS have generally been limited to (e) CALI/FALI approaches, which approximate bolus dosing, although they can be restricted to particular proteins or cellular compartments. (f) By contrast, the only available RES delivery strategy, T-REX, more closely mimics endogenous electrophile signaling and is capable of evoking a specific signaling response triggered by on-target electrophilic modifications.