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. Author manuscript; available in PMC: 2017 Sep 11.
Published in final edited form as: ACS Chem Biol. 2015 Nov 5;11(1):25–30. doi: 10.1021/acschembio.5b00439

Figure 3.

Figure 3

Streptavidin recruitment to PG-bound biotin requires WTA depletion. WT S. aureus Newman was labeled with K(biotin)-LPETG, K(biotin)-EGTLP, or DMSO control with or without 0.1 μg mL−1 tunicamycin and then incubated with Alexa 647-conjugated streptavidin. Protein recruitment was assessed by measuring total cell FL-4 fluorescence (from Alexa 647) on a flow cytometer. Experiment performed in technical duplicate; error bars indicate SEM. ** denotes a P value <0.01. Abbreviations: TCM, tunicamycin; WTA, wall teichoic acids.