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Entry and replication of Edwardsiella tarda in RAW 264.7. (A) RAW264.7 was incubated with E. tarda TX1G, and infection of the bacteria into RAW264.7 was observed continuously for 200 s with a confocal microscope. Arrow indicates bacteria. (B) RAW264.7 was infected with TX1G for 2h (a); the cells were then fixed, and extracellular TX1G was detected with rhodamine-labeled antibody (c). d merge of a–c.
