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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Inflammation. 2017 Apr;40(2):645–656. doi: 10.1007/s10753-017-0511-y

Figure 2. PLCβ-2-3′UTR Regulation of Luciferase Reporter Gene Expression by LPS.

Figure 2

To test the role of PLCβ-2 3′UTR in the destabilization of PLCβ-2 mRNA by LPS, a luciferase reporter assay was performed using two different plasmid constructs. The first contained the PLCβ-2 3′UTR downstream of the luciferase gene, and the other the GAPDH 3′UTR down-stream of the luciferase gene to serve as control. RAW264.7 cells were transfected with these constructs and treated with LPS for various time-periods. One set of wells was left untreated to provide constitutive luciferase expression. All samples were harvested at 24 hours and luciferase assays were performed using equal volumes of lysates and Renilla Glo. The results show the relative expression of luciferase from the PLCβ-2 3′UTR construct versus that of the GAPDH 3′UTR construct, and are the means ± S.D. of two separate experiments, each performed in triplicate. (* P_ <0.01 in comparison to Untreated Control)