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. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Inflammation. 2017 Apr;40(2):645–656. doi: 10.1007/s10753-017-0511-y

Figure 4. LPS induces Expression of TTP in RAW264.7 Cells and Murine Peritoneal Macrophages (MPMs).

Figure 4

RAW264.7 cells and MPMs from C57Bl/6J mice were treated with LPS (100ng/ml), or with RPMI-1% FBS alone as control (C) for various time periods. Following treatment, media were removed, cells were solubilized in SDS-polyacrylamide gel electrophoresis sample buffer and lysates (50μg protein/sample) were Western-blotted with TTP antibody. GAPDH was used as a loading control. Each experiment was performed twice with similar results. A typical Western blot for each cell type is shown. A: RAW264.7 cells. B: Mouse peritoneal macrophages. Band intensities were quantified by using ImageQuant analysis program (GE Health care).