Skip to main content
. Author manuscript; available in PMC: 2018 Apr 1.
Published in final edited form as: Inflammation. 2017 Apr;40(2):645–656. doi: 10.1007/s10753-017-0511-y

Figure 5. Stability of PLCβ-2 mRNA in Macrophages from WT and TTP−/− Mice.

Figure 5

A. PLCβ-2 mRNA levels in mouse peritoneal macrophages from wild type and TTP−/− mice were determined by Q-RT-PCR following treatment of macrophages with LPS (100ng/ml) for 0, 1.5, 4 and 8 hours. RNA was extracted from each sample and analyzed by Q-RT-PCR. mRNA levels were normalized to those of endogenous cyclophilin-D as a house-keeping gene. The results are the means ± S.D. of at two separate experiments, each performed in triplicate.

B. Macrophages from wild-type and TTP−/− mice were treated with actinomycin-D for 2 hours, followed by treatment with LPS in the presence of actinomycin-D. PLCβ-2 mRNA levels were determined as described in A. above.