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Nvbra expression in uninjected control and Nvbra/Cas9 embryos. In situ hybridization showing Nvbra expression in (A) uninjected embryos and (B) sibling embryos injected with Nvbra gRNAs and Cas9 (Nvbra/Cas9 embryos). Most control embryos show characteristic staining around the oral pole. Most Nvbra/Cas9 embryos show no staining; some show a smaller region of staining, whereas only a few show the normal staining pattern.
