Fig. 6.

Effects of Nvbra on the blastoporal gene regulatory network. Gene regulatory relationships in the endomesodermal GRN described by Röttinger et al. (2012). Because Nvbra is a transcription factor, we assume that Nvbra can directly affect only genes in its expression domain (the central ring). Effects on domains outside the central ring are assumed to be mediated by signaling molecules; indirect effects are indicated by the broken lines between regions of the embryo. Dotted arrows indicate interactions inferred from qPCR. Solid arrows indicate interactions inferred from both qPCR and in situ hybridization. Genes analyzed by in situ hybridization alone are not included. Upper panel: 24 h. Lower panel: 48 h. Genes are arrayed according to their normal expression domain at 24 h. Genes closer to the top of the diagram are transcribed earlier in development; those at successively lower positions are transcribed later (Röttinger et al., 2012).